QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 3. fkh2⁺ and sep1⁺ share an overlapping function. (A) sep1 ${Delta}$ rescues the lethal phenotype caused by overexpression of fkh2⁺. pREP3X:fkh2⁺ and pREP3X were transformed into wild-type and sep1 ${Delta}$ cells, and colonies streaked on EMM agar to induce overexpression of fkh2⁺. Micrographs of wild-type and sep1 ${Delta}$ cells overexpressing fkh2⁺, grown on EMM agar. Bar, 30 µm. (B) Loss of periodic transcription in sep1 ${Delta}$ cells. sep1 ${Delta}$ cdc25-22 (A 62) cells were synchronised by transient temperature arrest and samples taken every 15 minutes for northern blot analysis after release to the permissive temperature. The blot was probed consecutively with fkh2⁺, slp1⁺, ace2⁺, cdc15⁺, spo12⁺, fin1⁺ and adh1⁺ probes, the latter as a loading control. Quantification of each transcript against adh1⁺ is shown.

Return to article