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Fig. 5. S. pombe mbx1⁺. (A) Schematic representation of the predicted 436 amino acid S. pombe Mbx1p, MADS box-like protein (SPBC19G7.06). Position of the MADS box motif (M; 20-80) is shown. Phylogenetic tree showing the relationship between S. cerevisiae and S. pombe MADS box-like polypeptides, based on multiple sequence alignment, generated using the DNA cluster method in the Megalign programme based on a Jotun Hein algorithm (DNASTAR). (B) Fluorescent micrographs of mbx1 ${Delta}$ (JM 2331) cells, growing exponentially in EMM at 28°C. DNA stained with DAPI and septa marked with calcofluor. Representative images illustrating cytokinetic defects are shown. Bar, 10 µm. (C) Genetic interaction between mbx1⁺ and fkh2⁺. fkh2 ${Delta}$ sep1 ${Delta}$ (GG 631), fkh2 ${Delta}$ mbx1 ${Delta}$ (GG 551), sep1 ${Delta}$ mbx1 ${Delta}$ (GG 543) and sep1 ${Delta}$ fkh2 ${Delta}$ mbx1 ${Delta}$ (GG 754) cells were streaked on YE agar and grown at 28°C for 3 days. (D) sep1 ${Delta}$ , fkh2 ${Delta}$ and mbx1 ${Delta}$ show synthetic cytokinetic defects. Micrographs of cells growing in liquid EMM at 28°C: wild-type (GG 1), fkh2 ${Delta}$ (JM 2286), mbx1 ${Delta}$ (JM 2331), sep1 ${Delta}$ (GG 515), fkh2 ${Delta}$ mbx1 ${Delta}$ (GG 551), fkh2 ${Delta}$ sep1 ${Delta}$ (GG 631), sep1 ${Delta}$ mbx1 ${Delta}$ (GG 543) and fkh2 ${Delta}$ mbx1 ${Delta}$ sep1 ${Delta}$ (GG 754) were shown. Bar, 10 µm.

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