QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 6. Mbx1p is a component of PBF and interacts with Fkh2p. (A) Effect of gene deletions on PBF binding activity. Gel retardation analysis was performed using a cdc15⁺ promoter fragment as labelled probe with 20 µg of protein extracts from wild-type (GG 1), sep1 ${Delta}$ (GG 515), fkh2 ${Delta}$ (JM 2286), mbx1 ${Delta}$ (JM 2331), fkh2 ${Delta}$ sep1 ${Delta}$ (GG 631), fkh2 ${Delta}$ mbx1 ${Delta}$ (GG 551) and sep1 ${Delta}$ mbx1 ${Delta}$ (GG 543) cells are shown. Lane `f' indicates free probe. (B) Effect of gene tags on PBF binding activity. Gel retardation analysis was performed with protein extracts from wild-type (GG 1) cells or protein extracts from mbx1-13myc (JM 2349), fkh2-3HA (JM 2257) and fkh2-13myc (JM 2252) cells, either without plasmid (-) or transformed with a plasmid expressing a genomic copy of the equivalent wild-type gene (g), or transformed with a control plasmid (p). Lane `f' indicates free probe. (C) mbx1⁺ is not required for periodic transcription in M phase. cdc25-22 (GG 308) and mbx1 ${Delta}$ cdc25-22 (JM 2332) cells were synchronised by transient temperature arrest, and upon release to the permissive temperature samples taken every 15 minutes for northern blot analysis. The blot was probed consecutively with fkh2⁺, slp1⁺, ace2⁺, cdc15⁺, spo12⁺, fin1⁺ and adh1⁺ probes, the latter as a loading control. Quantification of each transcript against adh1⁺ is shown. Septation indices were counted microscopically and are shown in black (mbx1 ${Delta}$ cdc25-22) or grey (cdc25-22).

Return to article