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Fig. 7. Contagious nuclear apoptosis is suppressed by Bcl-2 and vMIA overexpression. Either HeLa Env or CD4 were transiently transfected with a vector expressing the neomycin resistance cassette only (Neo), Bcl-2, vMIA or p35. 24 hours after transfection, the HeLa Env cells were subjected to the standard STS pulse (3 h, STS), washed and cultured alone for 18 hours (3 h STS + 18 h) or co-cultured with untreated HeLa CD4 cells (contagion). The frequency of PACC, NA and 
m loss was assessed as in Fig. 6B, and results are given as means of three independent experiments. P values were calculated with the paired Student's t-test with respect to Neo controls untreated controls, #P<0.005.