QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 8. CD4⁺ T cells undergo contagious apoptosis both in vitro and in vivo. (A) Contagious, fusion-induced apoptosis occurring in T-lymphoid cell lines in vitro. Jurkat, CEM and U937 cell lines were pre-stained with CellTracker® Red or CellTracker® Green and co-cultured overnight with HeLa Env at a ration of 2:1. Env cells were pretreated for 3 hours in presence or absence of STS (3 µM) or ActD (5 µM). Loss of ${Delta}$ ${psi}$ m, PS exposure and plasma membrane permeability were assessed by cytofluorometric analysis, while gating on the CellTracker® pre-labeled population. Data are means of three experiments ± s.d. Significance was calculated with the paired Student's t-test with respect to untreated controls. *P<0.05; #P<0.005. (B) HeLa Env cells triggered contagious apoptosis of primary human T cells. Freshly drawn peripheral blood lymphocytes (PBL) or T lymphoblasts (obtained by 5days stimulation with PHA plus IL-2) were pre-stained with CellTracker® Red or CellTracker® Green, co-cultured overnight with CellTracker® Blue-pre-labeled HeLa Env cells and double-stained-positive cells were subjected to FACS analysis for ${Delta}$ ${psi}$ m loss and nuclear apoptosis. Prior to co-culture HeLa Env were given a r 3-hour incubation with STS or ActD (mean ± s.d., n=2, *P<0.05; #P<0.005), and the co-culture was performed in the presence or absence of 100 µM zVAD.fmk or 50 nM fusion inhibitor C34 peptide, as indicated. (C) HeLa Env cells trigger contagious apoptosis of Jurkat cells in vivo. CellTracker® Red-pre-labeled Jurkat cells were injected into the peritoneal cavity of athymic mice (nu/nu). HeLa Env were left untreated or pulsed with STS (3 µM), washed five times, and injected 8 hours after the Jurkat cells. After overnight incubation, peritoneal cells were recovered, and Jurkat cells (CellTracker® Red⁺) were analyzed cytofluorometrically to determine the ${Delta}$ ${psi}$ _m dissipation and PS exposure. Results are means for a minimum of five animals per group. *P<0.05; #P<0.005.

Return to article