QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 1. Determination of muMSC frequency in the bone marrow (A) and persistence of hematopoietic cell contamination in MSC cultures (B). (A) Representative FACS plots analysis of NOD/SCID BMMNCs (top plots) stained with CD45/CD11b/TER119/CD31 antibodies; the frequency of muMSCs in the bone marrow can be considered as the negative fraction for CD45 (R1) with the combination of the other cell surface antigens used here. Alternatively, the same frequencies can also be determined by using the following antibody combination: CD45/Lin/CD31 (bottom plots). By using this criterion no differences in the frequency was found between the three mouse strains studied. (B) The presence of CD45⁺/CD11b⁺ cells in different passages (P1-P3) of MSC cultures is shown (top plots). Percentages indicate the CD45^–/CD11b^– populations, which were considered as the MSC fraction. The CD45⁺/11b⁺ hematopoietic cell population expressed SCA-1 and different levels of CD34 and CD90 (middle and bottom plots, respectively). Results shown are mean±s.d from three independent experiments.

Return to article