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Fig. 2. In vitro eGFP expression by muMSCs following lentiviral transduction. A purified highly enriched muMSC population (puP4) was transduced with lentivirus for 20 hours. Analysis for eGFP expression was performed 1, 3 and 5 days post-transduction. (A) A representative overlay FACS plot from day 1 with m.o.i.=5 (solid line), 10 (blue dotted line), 30 (red dashed line), 50 (dark grey area) and untransduced control MSCs (light grey area) is shown. Cells gated in R1 expressing high levels of eGFP from different time points of analysis were plotted in the graph (B). Results shown are mean±s.d. from two independent experiments, and each time point was performed in duplicate.





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