QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 3. In vitro differentiation of eGFP-MSCs. Unstimulated eGFP-MSCs were viewed under UV light (A). After 14 days of induction, cells were fixed and stained with Oil Red O (B,C). Chondrogenic differentiation was revealed with Safranin O staining (E) with a representative section of the micropellet viewed under the fluorescent microscope before the staining (D). The osteogenic potential of MSCs was determined by staining for alkaline-phosphatase (F) and calcium production (G): stimulated eGFP-MSCs (black bar), non-stimulated eGFP-MSCs (grey bar), control-water (light grey bar). Results shown are mean±s.d. from two independent experiments, each one performed in triplicate. eGFP expression alone on myocyte-like cells (H). Myogenic differentiation was confirmed by staining with dystrophin-Cy3 (I,J) and FTM-Cy3 (K). eGFP expression alone on astrocyte-(L) and neuronal-like (O) cells. Neuronal differentiation was confirmed by staining with GFAP-Cy3 (M,N) and Tau-TRITC (O). Overlay of eGFP and dystrophin (J), eGFP and FTM (K) and eGFP with GFAP (N). Cells were counterstained with hematoxylin (B,C,F), DAPI (H-J, L-N) or methyl green (E). Magnifications: x100 (A,B,F); x200 (D,L-N); x400 (C,E,H-K,O).

Return to article