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Fig. 6. Contribution of eGFP-MSCs into myofibroblast and myofiber-like cells in vivo. (A) eGFP positive cells with myofibroblast morphology were located among skeletal muscle fibers (A1,4; brown). No expression of desmin was observed (A2; red staining) showing mutual exclusion of expression when double staining for desmin and eGFP was performed (A3). These same eGFP+ cells (brown) stained positive for {alpha}-sma (red) in serial sections (A5) resulting in a red-brown color. (A6) The same section stained for both rabbit and mouse immunoglubulins (isotype control) (A7). (B) Representative section of the skin from the ear with myofibers staining positive for eGFP (B2,3; brown cells). The same group of myofibers demonstrating expression of desmin (B4,5; pink-red staining) and FTM (B6,7; red) alone or in combination with an eGFP antibody (blue) giving a purple color (B5,7). Sections were counterstained with hematoxylin (except for B5,7). Magnifications: x200 (A7, B1,2,4-7); x400 (A1-6, B3).





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