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Fig. 4. Subcellular localization of the GFP-GMT tail mutants in the S. cerevisiae cells by indirect immunofluorescence. The GFP-tagged GMT tail mutant genes were expressed from the original VIG4 promoter on a CEN ARS plasmid in the wild-type cell. The cells were fixed with formaldehyde and observed by indirect immunofluorescence using anti-GFP antibody. The C-terminal sequences are shown. For expression of GFP-tagged GMT mutant derivatives, the plasmids used were pMA89 (A), pMA86 (B), pMAV587 (C), pMAV594 (D), pMAV595 (E), pMAV597 (F), pMAV599 (G) and pMAV601 (H).





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