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Fig. 6. Subcellular localization of the GFP-GMT-Wbp1p chimeric protein, which exchanges the C-terminal peptide of the GMT tail for that of Wbp1p. This chimeric protein is tagged with GFP and produced by VIG4 promoter and CEN ARS plasmid (pMAV592) in the wild-type cell. (A) The cells were fixed with formaldehyde and observed by indirect immunofluorescence using anti-GFP antibody (left) or by phase contrast microscopy (right). (B) Activity staining of invertase in 7.5% SDS-PAGE of the periplasmic fraction of the wild-type and vig4-1 transformant cells.





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