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Fig. 7. Localization of the mutant Emp47p with modified C-terminus. Cells were stained by immunofluorescence using anti-Myc and anti-Mnn9 antibodies to visualize Emp47p (myc-Emp47) and the Golgi mannosyltransferase (Mnn9), respectively. Merged images for phase-contrast microscopy are shown at the right. (A) The wild-type Emp47p (-RIRQEIIKTKLL). (B) A truncated Emp47p (-RIRQEII). (C) Addition of the GMT C-terminal peptide to the truncation (-RIRQEIIGSKQKKQQAQPLRK). (D) A C-terminal replacement with the GMT C-terminal peptide (-GSKQKKQQAQPLRK).





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