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Fig. 3. Time-lapse microscopy of T. gondii expressing a histone 2b-YFP fusion protein. At time zero, eight parasites in one vacuole have synchronously begun karyokinesis, revealed as a lobulation of the chromatin in the epifluorescence image (also discernible by phase contrast, arrow). Nuclear division is completed in some nuclei by 20 minutes and in all by 30 minutes (not shown). Cytokinesis is discernible by phase contrast approximately 15 minutes after the completion of nuclear division (45 minute time point) and is completed within 15 minutes (not shown).





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