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Fig. 8. Cell division in extracellular parasites. Parasites expressing IMC1-YFP were harvested after lysing out of host cells and suspended in culture medium. A droplet of parasite suspension was put on a coverslip, and a cell in mitosis (orange oval) was identified under epifluorescence observation. Other parasites in suspension moved in and out of the field of view during the observation period. For reference, two additional stationary parasites (interphase) are indicated by the yellow asterisks. Images were recorded every 5 minutes for 30 minutes, during which time the parasite in the orange oval progressed from mid-mitosis through completion of cytokinesis to form two daughter cells.





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