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Fig. 2. Effect of CAL on microneme secretion in T. gondii. (A) Western blot of parasite supernatants following stimulation of secretion at 37°C showed that secretion of MIC2 and MIC4 were increased by treatment with CAL. Stimulation with 1% ethanol was used as a positive control for secretion. Supernatants were compared to diluted lysates of cell standards (% pellets). Accidental parasite lysis was less than 1% as monitored by actin release. Identical blots were probed with monoclonal antibody 6D10 (cellular MIC2 and secreted MIC2; upper panel), rabbit polyclonal serum to MIC4 (middle panel) and rabbit polyclonal serum to actin (lower panel). (B) Phosphorimager analysis of western blots demonstrated that the amount of MIC2 and MIC4 secretion increased with increasing concentrations of CAL. Bars represent the average of four experiments (mean±s.e.m.). *A significant difference compared to DMSO levels, P<0.05; two-tailed Student's t-test.





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