QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 4. Effect of CAL on intracellular calcium levels. (A-C) Parasites were loaded with fura-2/AM as described in Materials and Methods, and suspended in buffer A containing 1 mM CaCl₂ (A) or 1 mM EGTA (B,C). (A) Increasing the concentration of CAL (added where indicated by the arrow) stimulated progressively greater increases in the amount of intracellular calcium when parasites were in buffer containing calcium. (B) CAL was added where indicated by the arrow at the concentrations shown to the right. Incubating parasites in the buffer containing EGTA promoted increases in intracellular calcium at low drug concentrations but decreased intracellular calcium at higher concentrations of CAL. (C) 1 µM CAL (b) or 2.5 µl DMSO (a) were added where indicated by the first arrow and 2 mM CaCl₂ was added where indicated by the second arrow. CAL-stimulated release of Ca²⁺ from intracellular stores led to Ca²⁺ entry from the extracellular medium. The increase in calcium detected with solvent alone was also observed in its absence and reflects the presence of some extracellular fura-2.

Return to article