spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. Intracellular Ca2+ mobilization is associated with eosinophil cytosolic alkalinization. Purified human eosinophils were loaded with BCECF/AM and stimulated with thapsigargin (10 µM), ionomycin (2 µM) and EGTA (5 mM) at 37°C. Fluorescence was measured over 90 minutes and was converted to pHi using a standard curve as described in Materials and Methods. Data are shown as mean±s.e.m. Thapsigargin ({circ}; n=3) and ionomycin ({blacksquare}; n=4) stimulation induced significant cytosolic alkalinization. Stimulation with ionomycin after EGTA pre-treatment ({square}; n=4) resulted in complete inhibition of alkalinization and induced significant acidification relative to pHi at time 0. Initial pHi values for thapsigargin, ionomycin and ionomycin + EGTA data sets were all 7.1±0.1. *P<0.05; **P<0.01 (compared to pHi at time 0 or, when labeling the bracket, comparing two final pHi values from different stimuli); n.s., not statistically significant.





Right arrow Return to article