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Fig. 3. PAF, but not IL-5, stimulation of human eosinophils increases [Ca2+]i. Purified eosinophils were loaded with Fura-PE3/AM, plated onto glass coverslips at a concentration of 0.25x106/ml and allowed to attach to the glass before imaging at room temperature. Data are shown as mean±s.e.m. (A) Addition of PAF (3 µM) produced a rapid rise in [Ca2+]i that exhibited diminishing oscillations with time in the continuous presence of PAF (n=6, using cells from two isolations). (B) Eosinophils treated with IL-5 (0.2 µM) failed to show any increase in [Ca2+]i. Subsequent stimulation with 3 µM PAF produced an increase in [Ca2+]i indicating that the cells were capable of responding to calcium-mobilizing agonists (n=7, using cells from two isolations).





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