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Fig. 7. Eosinophils express V-ATPase subunit E in whole cell lysates. Eosinophil whole cell lysates were prepared and examined for V-ATPase subunit E by western blotting as described in Materials and Methods. Commercially available mouse brain extract was used as a positive control (+). Shown are representative control (nIgG) and test (V-ATPase E) blots from three independent experiments using eosinophils. Each Eo Lysates lane represents an eosinophil extract from a separate donor. A band specific to the V-ATPase E blot is seen around 30 kDa in the positive control and in each eosinophil extract lane (arrow). The non-specific band around 48 kDa in the eosinophil extracts is probably eosinophil peroxidase (EPO).





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