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Fig. 8. The V-ATPase subunit E is localized to granular compartments in eosinophils. Eosinophils were isolated and stained for the V-ATPase subunit E and were analyzed by confocal microscopy as described in Materials and Methods. Shown are representative images from four independent experiments. Original images were taken at a magnification of x630. (A) Eosinophils stained with an isotype control antibody followed by FITC-conjugated secondary antibody. (B) Eosinophils stained with anti-V-ATPase subunit E followed by FITC-conjugated secondary antibody show a granular staining.





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