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Fig. 3. TRAP1-RNAi cells showing a significant defect in prestarvation response. (A) TRAP1-RNAi cells cultured for 2 days in the absence of tetracycline, and parental MB35 cells were separately harvested, starved and plated in 24-well plates at 5x10⁵ cells/cm². This was followed by incubation for the indicated times at 22°C. Bars, 200 µm. (B) Expression patterns of prestarvation genes discoidin I, car1, dia1 and dia3 in TRAP1-RNAi cells and MB35 cells. TRAP1-RNAi cells cultured for 2 days in the absence of tetracycline, and MB35 cells were separately harvested at the indicated cell density or stationary growth phase (1.5-2.0x10⁷ cells/ml, S), followed by extraction of total RNAs. The RNAs (40 µg for each) were size-fractionated and analyzed by northern blots using the cDNA probe of each gene. (C) TRAP1-RNAi cells cultured axenically in the absence of tetracycline for 2 days, and parental MB35 cells were spotted on the bacterial lawn, and incubated for 2 days at 22°C to allow them to develop. Bar, 1 mm (top panels), 500 µm (lower panels).

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