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Fig. 5. A novel prestarvation response. (A) Gß-null and PKAcat-null cells grown at 3-5x10⁶ cells/ml were fixed and immunostained with the Dd-TRAP1 antibody. In both transformants, Dd-TRAP1 is translocated to mitochondria, thus indicating that neither heterotrimeric G proteins nor PKA are required for the Dd-TRAP1 translocation. Bar, 5 µm (B) CGM was prepared from Ax-2 cells that had been grown up to a high cell density (8x10⁶ cells/ml). As control cells, growth-phase Ax-2 cells at a low cell density (5x10⁵ cells/ml) were harvested and resuspended in fresh growth medium (FGM) or CGM at 5x10⁵ cells/ml. After 3 hours of incubation, cells were fixed and immunostained with the Dd-TRAP1 antibody. In another experiment, cells were incubated in the CGM, either pretreated with protease (30 µg/ml of pronase E) (CGM/protease) or boiled for 15 minutes (+CGM/heat). Bar, 5 µm.

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