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Fig. 6. Delayed differentiation of TRAP1^OE cells and TRAP1^OE/dia1^– cells. (A) TRAP1^OE and parental Ax-2 cells grown at 1-2x10⁶ cells/ml were separately harvested, starved and plated either in a 24-well plate at 5x10⁵ cells/cm² (a-d) or on non-nutrient agar at 2.5x10⁵ cells/cm² (e-h). This was followed by incubation for the indicated times at 22°C. Bars, 200 µm (a-d); 500 µm (e-h). (B) Expression patterns of car1, dia3, lagC, dia1 and csA in starved TRAP1^OE cells. At the indicated times of starvation, total RNAs were extracted from TRAP1^OE and parental Ax-2 cells. The RNAs (20 µg for each) were size-fractionated and analyzed by northern blots using the cDNA probe of each gene. In starved TRAP1^OE cells, dia1 and csA genes are expressed early, while the expression patterns of car1, dia3 and lagC genes are rather delayed. (C) dia1^– and TRAP1^OE/dia1^– cells grown at 1-2x10⁶ cells/ml were separately harvested, starved and plated either in a 24-well plate at 5x10⁵ cells/cm² (a-d) or on non-nutrient agar at 2.5x10⁵ cells/cm² (e-h). This was followed by incubation for the indicated times at 22°C. Bars, 200 µm (a-d); 500 µm (e-h).

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