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Fig. 8. Loss of Dd-TRAP1 functions by introduction of the Dd-TRAP1 antibody into cells. (A) To introduce the Dd-TRAP1 antibody into Ax-2 cells, cells grown at 1x10⁶ cells/ml were harvested and electroporated with the antiserum raised against Dd-TRAP1 (anti-TRAP1 serum). As controls, cells were also electroporated with non-immune serum (non-immune serum) or without any serum (control). After electroporation, cells were incubated in BSS for the indicated times to allow differentiation. Bar, 250 µm. (B) Ax-2 cells grown at a low density (1x10⁶ cells/ml) were harvested and electroporated with or without the anti-TRAP1 serum. This was followed by incubation in BSS for 6 hours at 22°C and subsequent immunostaining with the Dd-TRAP1 antibody. Arrowheads indicate the Dd-TRAP1 remained in the cell cortex. Bar, 5 µm. (C) Ax-2 cells grown at a high density (1-1.5x10⁷ cells/ml) were harvested and electroporated with or without the anti-TRAP1 serum. This was followed by incubation in BSS for the indicated times under submerged conditions. Bar, 250 µm.

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