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Fig. 1. S. typhimurium, but not E. coli transcytoses flagellin across polarized epithelia. (A) T84 model epithelia were apically colonized with 5x108 CFU S. typhimurium (SL3201) or a flagellated commensal E. coli strain as described in Materials and Methods. At the indicated times, the apical or basolateral media was isolated and assayed for flagellin by immunoblotting. Apical supernatants were diluted 100-fold and basolateral supernatants were concentrated 20-fold. (B) MDCK model epithelia were colonized as in panel A. Basolateral supernatants were assayed as above for flagellin at the indicated time points. (C) Known concentrations of flagellin were subjected to a similar analysis to provide quantitative data. All data are representative of several experiments.





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