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Fig. 4. Transcytosis of flagellin is blocked at 20°C. T84 model epithelia were treated in a 37°C incubator or at ambient room temperature (observed to vary between 18.7 and 20.4°C). (A,B) Epithelia were colonized by 5x108 CFU S. typhimurium. Flagellin transcytosis (A) and bacterial invasion (B) were measured as described in Fig. 1 and Materials and Methods. (C) 10 mg/ml FITC-dextran (40 kDa) was added to the apical surface and its appearance was measured in the basolateral reservoir. 
, 37°C; 
, room temperature.
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