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Fig. 7. Altered levels/localization of flagellin in epithelia infected with SPI-2 deficient S. typhimurium. MDCK model epithelia were colonized with wild-type S. typhimurium (SL1344) or isogenic mutants for 1 hour, after which epithelia were paraformaldehyde-fixed, immunostained and analyzed by confocal microscopy. Actin is shown in purple. Salmonella complete surface antigens (CSA) are shown in red. Flagellin is shown in green. Flagellae stain with both CSA and flagellin antibodies and thus appear yellow. The sub-apical planes 1.5 µm and 3 µm below the apical surface are shown. Magnification x1000.





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