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Fig. 8. S. typhimurium SPI-2 mutant localizes to the cytoplasm in polarized epithelia. T84 Model epithelia were colonized with wild-type S. typhimurium or isogenic SPI-2 mutant. Two hours following colonization, cells were fixed and analyzed by electron microscopy as described in Methods. Arrows indicate bacteria. Bar, 2.5 µm.
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