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Fig. 1. PEVK segments and fragments in skeletal muscle titin. (A) Motif layout of skeletal muscle titin PEVK domain (Greaser, 2001). White rectangles indicate PPAK motifs, and gray ones, polyE motifs. The boundaries of the PEVK segments and fragments expressed and studied in this work are indicated by segments with arrows on both ends. The pure polyE fragment was generated by tandem duplication of the region marked with a line with diamonds at both ends. (B) SDS-PAGE of the expressed and purified PEVK segments and fragments. (a) molecular weight standards, (b) PEVKI, (c) PEVKII, (d) PEVKIII, (e) PPAK, (f) polyE-rich and (g) pure polyE.





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