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Fig. 2. Interaction of PEVK segments with actin. (A) Effect of PEVK segments on in vitro acto-HMM motility. Velocity as a function of PEVKI, -II and -III segment concentration. Inset graph shows velocity as a function of log[PEVK segment]. The in vitro motility assay was carried out at a KCl concentration of 25 mM, HMM concentration of 40 µg/ml and ATP concentration of 1 mM. (B) Solid-state surface binding of F-actin by PEVK segments as a function of ionic strength (adjusted by varying KCl concentration). The total filament length per field of view at the initial concentration of 25 mM KCl, were 115 µm, 145 µm and 195 µm for the PEVKI, -II and -III segments, respectively. (C) Actin crosslinking assay with various PEVK fragments (i) PEVKI, (ii) PEVKII and (iii) PEVKIII. Bar, 20 µm.





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