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Fig. 3. Effect of calcium and Ca²⁺ regulatory proteins on the actin binding of PEVKII. (A) Solid-state surface binding of F-actin by PEVKII as a function of free Ca²⁺ concentration (pCa). (B) SDS-PAGE of isolated native thin filaments. Left lane, thin filament preparation; right lane, molecular weight standards. Tm, tropomyosin; TnT, troponin T; TnI, troponin I; TnC, troponin C. (C) Surface binding of native thin filaments by PEVKII. Only short filaments are observed, in accordance with the size of the native thin filaments ( $~$ 1 µm). (i) Negative control (BSA on the surface). (ii) Positive control (HMM-coated surface; pCa 3). (iii) PEVKII-coated surface; pCa 9. (iv) PEVKII-coated surface; pCa 3. Bar, 10 µm.

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