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Fig. 5. PEVK fragment–F-actin co-sedimentation assay. (A) SDS-PAGE of polyE-rich fragment-actin binding assay. S, supernatant; P, pellet. The total polyE concentrations for the S-P lane pairs in this assay were: 0.036, 0.048, 0.060, 0.072, 0.084 and 0.096 mg/ml from left to right of gel. (B) Bound PEVK fragment concentration as a function of total PEVK fragment concentration. Inset shows a magnified view of the low-concentration range.





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