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Fig. 3. The substructure of meiotic MICs as seen by DAPI staining. (a) Stage IV crescents sometimes appear split into two longitudinal subunits that are separated by a gap (arrow) and have dark cross bands, which gives them the misleading appearance of being composed of tandemly arranged bipartite units (either replicated chromosomes or bivalents). (b) A broken stage IV crescent, which reveals its true composition of multiple strands (arrows). (c) A stage II MIC with a DNA-rich region (brightly stained with DAPI) near one pole, striated as if consisting of individual strands, and less dense chromatin towards the opposite pole. (d) In this stage II MIC the chromatin strands seem to form loops (arrow). Bar, 5 µm.





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