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Fig. 4. Chromosome painting by BrdU incorporation. BrdU-labeled chromosomes (red) extend along their entire length of the MICs in stage II (a,b), stage III (c) and stage IV (d). This observation is compatible both with a pole-to-pole and a narrow hairpin-loop arrangement, but not with a tandem arrangement of chromosomes within the crescents. In stage V (e,f) and stage VI (g,h) MICs, and also in diplotene-diakinesis (j), the chromosomes occupy an increasingly compact area. In d and e, some regions of the BrdU-positive threads appear double (arrows). The nuclei shown in f and i seem to contain two labeled chromosomes. (j,k) Conjugating cells, one of which has a labeled crescent. In addition both MACs in j carry labeled DNA. In a-d, the head pole is on the left. The chromatin is stained with DAPI (blue). Bars, 5 µm (in i for a-i) and 10 µm (in k for j,k).





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