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Fig. 5. FISH of meiotic MICs. (a-d) Telomere FISH (yellow) signals are most prevalent at the DAPI-bright, DNA-rich pole of stage II nuclei (a,b). The black and white image in (a) shows the DAPI distribution within the nucleus shown on the left. (c) In stage III nuclei, telomere FISH delineates the head region, which is separated from the trunk by the DAPI-dull neck (for designation see Fig. 1a). (d) The telomeres in stage IV MICs are clustered in the region corresponding to the former head as can be seen by the adjacent DAPI-dull area. (e-l) FISH with locus-specific probes. A pair of Teth2 loci (red) on homologous chromosomes appear mostly separate in stages I to III nuclei (e-h) and associated or fused as a single dot in stage IV (i). The position of the FISH signal in anaphase I (j) configurations confirms that the probed locus occupies a region in the distal third of a chromosome arm [arrows between C (centromere) and T (telomere)]. (k) A macronucleus (at half the magnification) shows multiple copies of the locus highlighted by the Teth2 probe. (l) A second locus (Teth1 – green) on the same chromosome and 167 kb away from Teth2 (red) is always located closer to the neck region (DAPI-dull to the left) of the crescents. All MICs are oriented with the head pole to the left. Bar, 5 µm.

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