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Fig. 2. TrkB.FL-GFP and TrkB.T1-GFP are localized in the plasma membrane of hippocampal neurons and are targeted to dendrites and axons. (A) Hippocampal neurons, transfected with TrkB.FL-GFP (left) and TrkB.T1-GFP (right) expression plasmids at 8 days in vitro (DIV), showed a clear accumulation of GFP fluorescence at the plasma membrane (2 days after transfection). (B) Immunocytochemistry of transfected neurons with an antibody against the dendritic marker MAP2 (at 16 DIV, 8 days after transfection). TrkB.FL-GFP and TrkB.T1-GFP in hippocampal neurons (green), were targeted to both, dendrites (MAP2-positive, red) and axons (MAP2-negative, arrows). Filopodia (small protrusions) were restricted to dendrites, whereas the appearance of axons was generally smooth. Scale bars: 5 µm (A); 20 µm (B).





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