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Fig. 5. Expression of TrkB.FL inhibits the TrkB.T1-induced growth of filopodia in a dominant negative fashion. Hippocampal microcultures were cotransfected with TrkB.T1-GFP and TrkB.FL-DsRed (relative amounts of DNA: 1:3). (A) A typical neuron, expressing both TrkB.T1-GFP and TrkB.FL-DsRed. Note the smooth dendritic surface, compared to B, a neuron expressing TrkB.T1-GFP only. (Insets in A an B show boxed areas at higher magnification.) (C) The reduction in the density of filopodia by coexpression of TrkB.FL is highly significant compared to controls expressing TrkB.T1 only (P<10–5 n=16-22 cells; three independent experiments). Both groups of neurons from a given experiment were located on the same glass cover slip. All experiments were performed in the presence of 200 nM K252a. Therefore, TrkB receptor tyrosine kinase activity is not required for the dominant negative action of TrkB.FL. Scale bars: 20 µm (A); 5 µm (inset).





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