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Fig. 7. Coexpression of a dominant negative p75^NTR variant (p75 ${Delta}$ ICD-DsRed) inhibits the TrkB.T1-induced growth of filopodia. Neurons were cotransfected with TrkB.T1-GFP and p75 ${Delta}$ ICD-DsRed (relative amounts of DNA: 3:1). (A) Typical neuron, expressing both, TrkB.T1-GFP and p75 ${Delta}$ ICD-DsRed. Note the lack of filopodia compared with the neuron expressing TrkB.T1-GFP only (on the same coverslip) shown in B. Insets in A and B show boxed areas at higher magnification. (C) The reduction in the density of filopodia by coexpression of p75 ${Delta}$ ICD-DsRed is highly significant (P<0.0001). (D) Neurons were cotransfected with GFP and p75 ${Delta}$ ICD-DsRed (relative amounts of DNA: 3:1). Cells coexpressing p75 ${Delta}$ ICD-DsRed showed a significant (P<0.0001) reduction of filopodia compared with neurons expressing GFP only, on the identical coverslips, highlighting the physiological significance of TrkB.T1-induced filopodial growth (n=13-30 cells from three independent experiments). Scale bars: 20 µm (A); 5 µm (inset).

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