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Fig. 5. Caspase activation is accelerated by anti-ß1-integrin neutralizing antibody. Young TA keratinocytes were either suspended in polypropylene or treated with anti-ß1-integrin neutralizing antibody (4 µg/ml). Cells were lysed at different time points and membranes blotted against anti procaspases-3, -6, -8, -9, -10 and Bid antibodies. ß-actin was used to normalize protein content (A). (B) Keratinocytes were treated with anti-ß1-integrin neutralizing antibody, as in A. Cytosolic and mitochondrial fractions were extracted from keratinocytes, as described in Materials and Methods, and blotted against anti-cytochrome c antibody.





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