QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 6. Caspase-8 inhibitor delays caspase activation and protects keratinocytes from anoikis. Keratinocytes were pretreated with caspase-9 inhibitor zLEDH-fmk and treated with anti-ß₁-integrin antibody. Cells were lysed at different time points and membranes were blotted against anti-procaspase-9, -8, -10 and -3 and anti-Bid antibodies. ß-actin was used to normalize protein content (A). (B) Keratinocytes were pretreated with caspase-8 inhibitor zIETD-fmk and treated with anti-ß₁-integrin antibody. Cells were lysed at different time points and membranes were blotted against anti-procaspase-8, -9 and -3 and anti-Bid antibodies. ß-actin was used to normalize protein content. (C) Keratinocytes were pretreated with caspase-8 inhibitor zIETD-fmk or with caspase-9 inhibitor zLEDH-fmk and either suspended in polypropylene or treated with anti-ß₁-integrin antibody. Apoptosis was evaluated by TUNEL staining, as described in Materials and Methods. Approximately 100 cells were evaluated, in randomly selected high-power fields, and the percentage of TUNEL-positive cells was counted. Each experiment was repeated three times. Results are expressed as the mean percentage ± s.d. from three experiments. Student's t test was used for comparison of the means.

Return to article