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Fig. 9. Anti-ß1-integrin fails to induce anoikis in c-FLIPL overexpressing keratinocytes. After 16 hours of treatment with anti-ß1 integrin, anoikis was measured by TUNEL. Approximately 100 cells were evaluated, in randomly selected high-power fields, and the percentage of TUNEL positive cells was counted. Each experiment was repeated three times. Results are expressed as the mean percentage ± s.d. from three experiments. Student's t test was used for comparison of the means (A). (B) Keratinocytes were fixed in situ with paraformaldehyde and stained by the TUNEL technique and revealed by converter alkaline phosphatase, according to the manufacturer's instruction.





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