spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. Transgenic Myo3A fusion protein localizes to the distal end of Xenopus rod inner segment actin filament bundles. The entire Myo3A protein fused to the C-terminal of GFP (GFP:Myo3A) was used to make transgenic Xenopus that express the transgene in rod photoreceptors using a modified Xenopus rod opsin promoter. (A) In a section from a GFP:Myo3A transgenic tadpole retina stained with Texas-Red phalloidin to visualize actin filament bundles, GFP:Myo3A (green) accumulates at the distal end of phalloidin-stained actin filament bundles (red). Arrows indicate the presence of the fusion protein at the distal end of actin filament bundles that protrude into the retinal pigmented epithelium (RPE). No fluorescence was detected in the rod nuclei (N). (B) Diagram of the structural features of rod photoreceptors and the overlying RPE layer in retinal sections of the photoreceptor. OS, outer segment; CP, calycal processes; IS, inner segment; CC, connecting cilium; N, nucleus; S, synapse. Actin filament bundles in photoreceptors extend from the inner segment and terminate in the calycal processes surrounding the proximal outer segment in rod photoreceptors. (C) A retinal section from a control GFP transgenic tadpole retina exhibits fluorescence in rod nuclear (labeled with DAPI, blue) and cytosolic (inner segment, synapse and connecting cilium) compartments of rods. The arrow in panel C indicates fluorescence in the connecting cilium. Scale bar: 10 µm.





Right arrow Return to article