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Fig. 3. Expression of the GFP:Myo3A transgene results in the formation of abnormally large rod calycal processes. Rods from a GFP:Myo3A transgenic retina contain thicker rod calycal processes (A,C, black arrows; E, white arrowheads) than those found in wild-type rods (B,D, arrows). In addition, a calycal process from a GFP:Myo3A rod protrudes into the RPE layer and extends beyond the normal distal boundary seen in wild-type rod calycal processes (C, arrow). Actin filament bundles are visible in a longitudinal section through an enlarged calycal process found in a GFP:Myo3A retina (C, inset). Cross-sections of rod outer segments from wild-type (D) and GFP:Myo3A transgenic tadpoles (E) contain numerous normal-sized calycal processes (white arrows) but two abnormally large calycal processes are observed in the GFP:Myo3A retina (white arrowheads). Actin filament bundles are present in rod calycal processes of a wild-type retina (F) and of the GFP:Myo3A retina (G; boxed region in E), but there are fewer actin filament bundles visualized in the wild-type calycal process compared with the enlarged calycal process in the GFP:Myo3A retina. Scale bars: (A-C) 2 µm; (inset in C) 0.2 µm; (D,E) 1 µm; (F,G) 0.25 µm.

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