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Fig. 4. GFP:Myo3A transgenic retinas contain rod calycal processes with increased cross-sectional area. Cross-sections of rods from two GFP:Myo3A, a wild-type and GFP control retina similar to those shown in Fig. 3D and E were used to measure the cross-sectional area of calycal processes from randomly chosen rods. For each rod, the calycal processes were categorized by size (cross-sectional area), and the average percentage of calycal processes per rod within a cross-sectional area range was plotted for each experimental and control tadpole retina. Note that the first three bins on the x-axis represent 0.01 µm² and together represent the size of the remaining six bins (0.03 µm²). Rod calycal processes with cross-sectional areas less than 0.03 µm² were seen in both control and experimental retinas, but only rods from the two GFP:Myo3A transgenic tadpoles contained calycal processes larger than 0.03 µm². In the wild-type and GFP control retinas most of the calycal processes had cross-sectional areas less than 0.01 µm² but a few were in the ranges 0.01-0.02 and 0.02-0.03 µm². Therefore, any calycal processes less than 0.03 µm² in cross-sectional area are within the normal range, while those greater than 0.03 µm² are defined as being clubs, since calycal processes of this size are never found in wild-type or control rods.

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