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Fig. 6. Expression of transgenic GFP:Myo3A causes rod cell death in the retina. Three to four 0.5 µm sections from transgenic and control tadpole retinas were counted to determine the average rod:cone ratio for retinas from GFP:Myo3A (black), GFP (gray) and wild-type (white) tadpoles. The average rod:cone ratio in GFP:Myo3A transgenic retina (1.06±0.020, n=18 tadpole retinas) was reduced compared with control GFP transgenic (1.62±0.020, n=15 tadpoles retinas) and wild type (1.78±0.056, n=14 tadpole retinas) retina. The reduction in the rod:cone ratio from GFP:Myo3A retinas compared with either GFP or wild-type retinas was statistically significant (P<0.001) presumably because of the death of rods expressing the Myo3A transgene. There was no difference in average rod:cone ratios of control transgenic GFP and wild-type retinas. Error bars, standard error of the mean.





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