QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 7. Motor activity and the Myo3A tail are required for Myo3A localization to the distal end of inner segment actin filament bundles. (A) A retinal section from a transgenic tadpole containing the GFP:Myo3A (motor-inactive) protein displayed fluorescence in the inner segment (IS), connecting cilium (arrow) and synapse (arrowhead). (B) Deletion of the Myo3A tail (GFP:Myo3A ${Delta}$ T) also resulted in fluorescence confined to the cytosol of the rod inner segment, connecting cilium and synapse. (C) Fluorescence from a GFP:Myo3A transgene with a deletion of the 22 amino acid 3THDII domain (GFP:Myo3A ${Delta}$ 3THDII) localized to inner segment actin filament bundles (arrow) but also displayed extensive levels of fluorescence in the inner segment cytosol and synapse. (D) In addition to cytosolic fluorescence, an accumulation of fluorescence from GFP:Myo3A ${Delta}$ 3THDII was also observed in the distal ends of actin filament bundles (arrows). None of the nuclei of transgenic retinas exhibited fluorescence (N, blue DAPI label). RPE, retinal pigmented epithelium; OS, outer segment. Scale bar: 10 µm.

Return to article