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Fig. 2. Developmental expression pattern of MOR23 in mouse testis. (A) In situ hybridization using protamine 1 or H1t antisense probe was performed in serial sections to determine the stages of the seminiferous tubes expressing MOR23. (B) Individual tubules are numbered in the image, and the presence (+) or absence (–) of MOR23, protamine 1 and H1t in the corresponding tubules are shown in the table. MOR23-encoding transcripts were observed in seminiferous tubules from which protamine-1-encoding transcripts were absent but H1t transcripts were present. (C) Expression patterns of MOR23 and H1t in the corresponding areas of serial sections. MOR23-encoding transcripts are localized in the round spermatid layer, whereas H1t-encoding mRNA was found in spermatocytes. (D) Diagram of the 12-stage growth cycle of mouse spermatogenesis (Russell, 1990) showing the stages expressing MOR23 (magenta), protamine 1 (blue) and H1t (green) transcripts. MOR23 was expressed during stages VI-VIII. Scale bar, 100 µm.





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