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Fig. 6. Effect of lyral on Ca²⁺ levels in spermatogenic cells and cauda epididymal sperm from MOR23 Tg and wild-type mice. (A) (left) A representative response of Tg mouse testis spermatogenic cells to increasing concentrations of lyral. (middle) A dose-response curve for lyral in spermatogenic cells from Tg (filled squares) and wild-type (filled triangles) mouse testis (± s.e., n=3). (right) The proportion of spermatogenic cells responding to 3 mM lyral in Tg and wild-type mice. ^**, P<0.01 (Student's t test, ± s.e., n=18). (B) (left) A representative response of an epididymal spermatozoon isolated from a Tg mouse to lyral and K8.6. (middle) Proportion of spermatozoa from Tg and wild-type mice that respond to 2.5 mM lyral. ^**, P<0.01 (Student's t test, ± s.e., n=6). (right) The average amplitude of the response to 2.5 mM lyral in Tg and wild-type mouse sperm. LY, lyral; WT, wild type.

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