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Fig. 3. Expression of mutant BRG1 causes a change in the size and distribution of focal adhesions but not in paxillin or actin protein levels. Immunostaining for (a,b) paxillin, (c,d) phalloidin, (e,f) the merged images of a,c and b,d, respectively, and (g,h) DAPI staining in B05-1 cells grown to approximately 50% confluence in the presence (a,c,e,g) or absence (b,d,f,h) of tetracycline for 3 days. Bar, 10 µm. (i) Western blot demonstrates that total cellular paxillin and actin levels are unchanged by tetracycline or expression of mutant BRG1 in three different cell lines. Flag immunoreactivity demonstrates that the mutant BRG1 was expressed.





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