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Fig. 3. Distribution of Golgi markers in HeLa cells expressing high levels of GCC185. HeLa cells were transiently transfected as indicated, with either GFP-GCC185 or myc-GCC185, fixed, permeabilized and co-stained for GM130, TGN46 or ß-COP. GM130 was detected with mouse monoclonal antibody and Alexa 568 goat anti-mouse IgG, TGN46 with sheep anti-TGN46 and FITC-donkey anti-sheep Ig, ß-COP with rabbit anti-ß-COP and Alexa 568 goatanti-rabbit IgG. Images of transfected cells expressing high levels of GCC185 and also superimposed images (merge) are shown. Control incubations demonstrated no cross-reactivity between the anti-Ig conjugates and the irrelevant primary antibody. Bars, 10 µm.





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